x

    Book Now

    Blog Details

    Prediction of Structure & Function of Helicobacter Pylori hypothetical protein MPG40_02440: An In-Silico approach

    Project Lead

    Sheikh Ambia

    Our Approach 

    We selected a hypothetical protein MPG40_02440(accession no-WP245064145.1): molybdopterin guanine dinucleotide-containing enzymes of   H. pylori for our study that supports virulence via energy production.

    Research Objectives / Goals

    Helicobacter pylori (H. pylori), a common gastric infection affecting more than half of the world’s population, presents diagnostic and therapeutic challenges for clinicians globally.
    Diagnostic Strategies: Researchers aim to enhance diagnostic accuracy by evaluating various testing methods. Factors such as patient age, symptoms, medication use, test reliability, availability, and cost influence the choice of diagnostic approach.
    Treatment Optimization: With rising antibiotic resistance, selecting effective therapies is crucial. Regional resistance patterns and prior antibiotic exposure guide treatment decisions. Bismuth quadruple therapy and alternative macrolide-containing regimens are considered. Untreated H. pylori is associated with serious complications, including peptic ulcer disease, mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. Ongoing research aims to improve diagnostic accuracy, optimize treatment, and prevent H. pylori-related complications. 

    Context

     Helicobacter Pylori belongs to a family of gram negative, spiral shaped bacteria that have the ability to damage the tissue of our stomach and the first part of small intestine (the duodenum) by producing urease enzyme that increase the pH and also weakens stomach’s lining that can lead to gastroduodenal diseases such as peptic ulcer, chronic gastritis, duodenal ulcer(DU), gastric metaplasia(GM), and gastric cancer.

    Impact

    The protein we selected for our study was molybdopterin guanine dinucleotide-containing enzymes of H. pylori cytoplasmic enzymes associated with energy production, thus supports virulence in pathogenic bacteria. Our study aimed to predict the structure, function, and binding site of this hypothetical protein(accession no-WP245064145.1), which will help to compare its homology with other proteins of bisC_fam superfamily and to enrich the list of potential proteins of the Helicobacter Pylori vaccine and drug candidates.

    Public Health Relevance Statement

    H. pylori – a flagellated microaerophilic gram-negative bacteria, present in the stomach of healthy individuals, asymptomatic in 50% of the world’s population.Gastric cancer is the fifth most common cancer worldwide, with approximately one million new cases per year.According to the WHO and International Agency for Research on Cancer, H. pylori is classified as a group 1 carcinogen.To prevent these condition its important to conduct more study on this micro-organism’s structure and functions that will improve the medical system.